CV

Curriculum Vitae of Intaek George Lee, PhD

(Last updated in Nov.2024)

 

Contact Information:

E-mail: info@anathapindikahealth.com

 

Nationality and Citizenship

US Citizen

 

Education:

  • 1998 B.A   University of California, Berkeley, Department of Molecular and Cell Biology, Berkeley, CA
  • 2005 Ph.D  University of Georgia, Department of Biochemistry and Molecular Biology, Athens, GA

 

Research and Professional experiences:

 

  • 8/1996 – 5/1997 Undergraduate research assistant, University of California, Berkeley, Department of Plant and Microbial Biology, Berkeley, CA (Norman Terry lab); worked under supervision of a postdoc on a phytoremediation project for removal of selenium using transgenic Arabidopsis thaliana.
  • 8/1998 – 5/2005 Ph.D program, Department of Biochemistry and Molecular Biology, University of Georgia,  Athens, GA (Adviser: Michael Pierce, Ph.D)
- Studies on effect of GnT-VB expression on NGF induced neurite outgrowth in PC12 cells.
- Studies on the interaction between β1 integrin and vacuolar H+-ATPase 16 kDa subunit in the endoplasmic reticulum
- Studies on the effect of increased β(1,6) branched N-linked oligosaccharides on β1 integrin function in the α5β1 integrin-mediated tumor cell motility on extracellular matrix

 

  • 06/2005 – 10/2008 NIH Postdoctoral fellow, Division of Hematology, Washington University School of Medicine, St.Louis, MO (Adviser: Stuart Kornfeld, M.D)
- Biochemical characterization on tri-partite interaction among cargo sorting motifs, activated Arf-1.GTP and clathrin adaptor AP-1
- Study on di-leucine-based cargo sorting signal-binding sites of AP-1
- The role of the γ-subunit of UDP-N-acetylglucosamine:N-acetylglucosamine-1-phosphotranferase in the mannose 6-phosphorylation of lysosomal enzymes.
 
  • 11/2008 – 10/2011 Postdoctoral associate, Department of Cell Biology, Yale University School of Medicine, New Haven, CT (Adviser: James Rothman, Ph.D)

- Study of Tether-SNARE interaction in Golgi biogenesis and anterograde secretory pathway in mammalian cells using electron microscopy, biochemistry and cell biology

 

  • 11/2011-01/2014 Associate Research Scientist, Department of Cell Biology, Yale University School of Medicine, New Haven, CT (Adviser: James Rothman, Ph.D)

 

  • 02/2014- 04/2020 Visiting Scholar, Department of Cell Biology, Yale University School of Medicine, New Haven, CT
 
  • 02/2014- 12/2021 Co-Principal Investigator and ShanghaiTech Fellow: Lab of Nanoscale Cell Biology, School of Life Science, ShanghaiTech University, Pudong, Shanghai, China

 

Research Skills:

Cell biology (various mammalian cell-based assays and kinase assays, etc), Protein biochemistry (recombinant protein expression/purification using mammalian, insect, bacteria, protein-protein interaction study), Molecular biology (PCR, RT-PCR, realtime PCR, cloning, epitope tagging, etc), BioID; Fluorescence microscopy (confocal, super-resolution, quantitative imaging and advanced live imaging), protein secretion; cell signaling; cancer biology.

 

Selected Publications

  1. Jie Jia, Lianhui Zhu, Xihua Yue, Shuocheng Tang, Shuaiyang Jing, Chuanting Tan, Yulei Du, Jingkai Gao, Intaek Lee† and Yi Qian† “Crosstalk between KDEL receptor and EGF receptor mediates cell proliferation and migration via STAT3 signaling”, Cell Communication and Signaling, 2024, 22:140 (†co-corresponding author). https://doi.org/10.1186/s12964-024-01517-w
  2. Jia Jia, Shuocheng Tang, Xihua Yue, Shuaiyang Jing, Lianhui Zhu, Chuangting Tand, Jingkai Gao, Yulei Du, Intaek Lee† and Yi Qian† “An A-Kinase Anchoring Protein (ACBD3) Coordinates Traffic-Induced PKA Activation At The Golgi”, 2023, May, 299(5): 1046696 Journal of Biological Chemistry, Editor's Pick (†Co-corresponding author) https://doi.org/10.1016/j.jbc.2023.104696
  3. Xihua Yue, Neeraj Tiwari, Lianhui Zhu, Hai Dang Truong Ngo and Jae-Min Lim, Bopil Gim, Shuaiyang Jing Yijing Wang, Yi Qian, andIntaek Lee† “Tankyrase-1-Mediated Degradation of Golgin45 Regulates Glycosyltransferase Trafficking and Protein Glycosylation In Rab2-GTP-Dependent Manner” Communications Biology, 2021, Dec 7; 7(4):1370 (†Corresponding author). https://doi.org/10.1038/s42003-021-02899-0. 
  4. Xihua Yue, Yi Qian, Lianhui Zhu, Bopil Gim, Mengjing Bao, Jie Jia, Shuaiyang Jing, Yijing Wang, Chuanting Tan, Francesca Botanelli, Pascal Ziltener, Sunkyu Choi, Piliang Hao and Intaek Lee†,“ACBD3 Modulate Protein Kinase A Activity on KDEL Receptor For its Retrograde Trafficking via Arf1-Dependent Tubulovesicular Carrier” BMC Biology, 2021 Sep 7;19(1):194 (†Corresponding author). https://doi.org/10.1186/s12915-021-01137-7.
  5. Jie Jia, Xihua Yue, Lianhui Zhu, Shuaiyang Jing, Yijing Wang, Bopil Gim, Yi Qian†, and Intaek Lee†, “KDEL Receptor Is a Cell Surface Receptor That Cycles Between the Plasma Membrane and the Golgi via Clathrin-Mediated Transport Carriers” Cellular and Molecular Life Sciences, 2021 Feb;78(3):1085-1100 (†Corresponding author). https://doi.org/10.1007/s00018-020-03570-3.
  6. Intaek Lee*,Balraj Doray*, Jennifer Govero and Stuart Kornfeld “Binding of Cargo Sorting Signals to AP-1 enhances its association with Arf1-GTP” Journal of Cell Biology 2008, 180:467-472 (*Equal Contribution) [Faculty of 1000]. https://doi.org/10.1083/jcb.200709037.
  7. Intaek Lee*†, Neeraj Tiwari*, MyunHwa Dunlop*, Morven Graham, Xinran Liu and James E. Rothman†“Membrane Adhesion Dictates Golgi Stacking and Cisternal Morphology” Proceedings of the National Academy of Sciences 2014, 111:1849-1854 (†Co-corresponding author) [Faculty of 1000]. https://doi.org/10.1073/pnas.1323895111.

 

 

Publications:

  1. Jingkai Gao, Yulei Du, Shuaiyang Jing, Lianhui Zhu, Xihua Yue, Intaek Lee† and Yi Qian† “Golgin45 Recruits Polo-like Kinase 1 to The Kinetochore and Regulates Kinetochore-Microtubule Attachment During Mitosis” 2024, under review (†Co-corresponding author).

 

  1. Shuaiyang Jing, Jingkai Gao, Neeraj Tiwari, Yulei Du, Lianhui Zhu, Bopil Gim, Yi Qian†, Xihua Yue† and Intaek Lee† “SUMOylated Golgin45 Associates with PML-NBs to Transcriptionally Regulate Lipid Metabolism Genes During Heat Shock Stress”, 2024, in press in Communications Biology (†Co-corresponding author). https://doi.org/10.21203/rs.3.rs-1485254/v1.

 

  1. Jie Jia, Xihua Yue, Shuaiyang Jing, Lianhui Zhu, Chuanting Tan, Yulei Du, Jingkai Gao, Shuocheng Tang, Intaek Lee† and Yi Qian† “Crosstalk Between KDEL Receptor and EGF Receptor Mediates Cell Proliferation and Migration Via STAT3 Signaling” Cell Communication and Signaling, 2024, 22:140 (†co-corresponding author). https://doi.org/10.1186/s12964-024-01517-w

 

  1. Chuanting Tan, Yulei Du, Shuaniyang Jing, Jingkai Gao, Lianhui Zhu, Yi Qian, Xihua Yue† and Intaek Lee† “KDEL Receptor Trafficking To The Plasma Membrane Is Regulated by ACBD3 and Rab4A-GTP”, 2023, Cells, 2023, April; 12(7), 1079 (†Corresponding author) https://doi.org/10.3390/cells12071079

 

  1. Jia Jia, Shuocheng Tang, Xihua Yue, Shuaiyang Jing, Lianhui Zhu, Chuangting Tand, Jingkai Gao, Yulei Du, Intaek Lee† and Yi Qian† “An A-Kinase Anchoring Protein (ACBD3) Coordinates Traffic-Induced PKA Activation At The Golgi”, 2023, May, 299(5): 1046696 Journal of Biological Chemistry, Editor's Pick (†Co-corresponding author) https://doi.org/10.1016/j.jbc.2023.104696

 

  1. Xihua Yue, Bopil Gim, Lianhui Zhu, Chuanting Tan, Yi Qian, Morven Graham, Xinran Liu and Intaek Lee† “Retro-2 Alters Golgi Structure” Scientific Reports, 2022 Sept; vol.12: 14975 (†Corresponding author). https://doi.org/10.1038/s41598-022-19415-x

 

  1. Xihua Yue, Neeraj Tiwari, Lianhui Zhu, Hai Dang Truong Ngo and Jae-Min Lim, Bopil Gim, Shuaiyang Jing Yijing Wang, Yi Qian, andIntaek Lee† “Tankyrase-1-Mediated Degradation of Golgin45 Regulates Glycosyltransferase Trafficking and Protein Glycosylation In Rab2-GTP-Dependent Manner” Communications Biology, 2021, Dec 7; 7(4):1370 (†Corresponding author). https://doi.org/10.1038/s42003-021-02899-0.  

 

  1. Xihua Yue, Yi Qian, Lianhui Zhu, Bopil Gim, Mengjing Bao, Jie Jia, Shuaiyang Jing, Yijing Wang, Chuanting Tan, Francesca Botanelli, Pascal Ziltener, Sunkyu Choi, Piliang Hao and Intaek Lee†,“ACBD3 Modulate Protein Kinase A Activity on KDEL Receptor For its Retrograde Trafficking via Arf1-Dependent Tubulovesicular Carrier” BMC Biology, 2021 Sep 7;19(1):194 (†Corresponding author). https://doi.org/10.1186/s12915-021-01137-7.

 

  1. Jie Jia, Xihua Yue, Lianhui Zhu, Shuaiyang Jing, Yijing Wang, Bopil Gim, Yi Qian†, and Intaek Lee†, “KDEL Receptor Is a Cell Surface Receptor That Cycles Between the Plasma Membrane and the Golgi via Clathrin-Mediated Transport Carriers” Cellular and Molecular Life Sciences, 2021 Feb;78(3):1085-1100 (†Corresponding author). https://doi.org/10.1007/s00018-020-03570-3.

 

  1. Jiannan Fan, Xuechen Liu, Feifei Mao, Xihua Yue, Intaek Lee, Yingke Xu,“Proximity Proteomics identifies novel function of Rab14 in trafficking of Ebola virus matrix protein VP40” Biochem Biophys Res Commun, 2020; 527(2); 387392. https://doi.org/10.1016/j.bbrc.2020.04.041.

 

  1. Neeraj Tiwari, Morven Graham, Xinran Liu, Xihua Yue, Lianhui Zhu, Dipak Meshram, Sunkyu Choi, Yi Qian, James E. Rothman and Intaek Lee†,“Golgin45-Syntaxin5 Interaction Contributes to Structural Integrity of the Golgi Stack” Scientific Reports 2019 Aug; vol. 9: 12465 (†Corresponding author). https://doi.org/10.1038/s41598-019-48875-x.

 

  1. Xihua Yue, Qian Yi, Bopil Gim and Intaek Lee†, “Acyl-CoA-Binding Domain-Containing 3 (ACBD3; PAP7; GCP60): A Multi-Functional Membrane Domain Organizer” International Journal of Molecular Sciences 2019 Apr 24;20(8):2028 (†Corresponding author); Review. https://doi.org/10.3390/ijms20082028.

 

  1. Xihua Yue, Mengjing Bao, Romain Christiano, Siyang Li, Jia Mei, Lianhui Zhu, Feifei Mao, Qiang Yue, Panpan Zhang, Shuaiyang Jing, James E. Rothman†, Yi Qian†, and Intaek Lee†, “ACBD3 Functions As a Scaffold to Organize the Golgi Stacking Proteins and a Rab33b-GAP” FEBS Letters 2017 Sep;591(18):2793-2802 (Editor’s Choice) (†Corresponding author). https://doi.org/10.1002/1873-3468.12780.

 

  1. Intaek Lee*†, Neeraj Tiwari*, MyunHwa Dunlop*, Morven Graham, Xinran Liu and James E. Rothman†“Membrane Adhesion Dictates Golgi Stacking and Cisternal Morphology” Proceedings of the National Academy of Sciences 2014, 111:1849-1854 (†Co-corresponding author) [Faculty of 1000]. https://doi.org/10.1073/pnas.1323895111.

 

  1. Intaek Lee*,Yi Qian*, Wang-Sik Lee, Mariko Kudo, William Canfield, Peter Lobel and Stuart Kornfeld “Functions of the alpha, beta, and gamma subunits of UDP-GlcNAc:lysosomal enzyme N-acetylglucosamine-1-phosphotransferase” Journal of Biological Chemistry 2010, 285:3360-3370 (*co-first author). https://doi.org/10.1074/jbc.M109.068650.

 

  1. Huabei Guo, Heather Johnson, Matthew Randolph, Intaek Lee and Michael Pierce “Knockdown of GnT-Va expression inhibits ligand-induced downregulation of the epidermal growth factor receptor and intracellular signaling by inhibiting receptor endocytosis” Glycobiology 2009, 19:547-559. https://doi.org/10.1093/glycob/cwp023. 

 

  1. Intaek Lee, Matthew Drake, Linton Traub and Stuart Kornfeld “Cargo sorting signals promote AP-1 polymerization in an Arf1-GTP-independent manner” Archives of Biochem and Biophysics2008, 479:63-68. https://doi.org/10.1016/j.abb.2008.08.009.

 

  1. Intaek Lee*, Balraj Doray*, Jennifer Govero and Stuart Kornfeld “Binding of Cargo Sorting Signals to AP-1 enhances its association with Arf1-GTP” Journal of Cell Biology 2008, 180:467-472 (*Equal Contribution) [Faculty of 1000]. https://doi.org/10.1083/jcb.200709037.

 

  1. Intaek Lee*, Balraj Doray*, Jane Knisely, Guojun Bu and Stuart Kornfeld “The γ/σ1 and α/σ2 Hemicomplexes of Clathrin Adaptors AP-1 and AP-2 Harbor the Di-leucine Recognition Site” Molecular Biology of the Cell2007, 18:1887-1896 (*co-first author). https://doi.org/10.1091/mbc.e07-01-0012. 

 

  1. Karen Abbott, Karolyn Troupe, Intaek Leeand Michael Pierce “Integrin-dependent neuroblastoma cell adhesion and migration on laminin is regulated by expression levels of two enzymes in the O-mannosyl-linked glycosylation pathway, PomGnT-1 and GnT-Vb” Experimental Cell Research 2006, 312:2837-2850. https://doi.org/10.1016/j.yexcr.2006.05.022. 

 

  1. Intaek Lee, Huabei Guo, Maria kamar, Jin-Kyu Lee, Gerardo Alvarez-Manilla and Michael Pierce “N-acetylglucosaminyltranferase VB expression enhances β1 integrin- dependent PC12 neurite outgrowth on laminin and collagen” Journal of Neurochemistry2006, 97:947-956. https://doi.org/10.1111/j.1471-4159.2006.03785.x.

 

  1. Huabei Guo, Intaek Lee, Beau Bryan, and Michael Pierce “Deletion of mouse embryo fibroblast N-acetylglucosaminyltransferase V stimulates α5β1 integrin expression mediated by the protein kinase C signaling pathway” Journal of Biological Chemistry2005, 280:8332-8342. https://doi.org/10.1074/jbc.m413532200. 

 

  1. Intaek Lee, Mhairi Skinner, Huabei Guo, Avinash Sujan and Michael Pierce “Expression of the vacuolar H+-ATPase 16 kDa subunit results in the triton X-100- insoluble aggregation of β1 integrin and reduction of its cell surface expression” Journal of Biological Chemistry2004, 279: 53007-53014. https://doi.org/10.1074/jbc.m405717200.

 

  1. Mika Kaneko, Gerardo Alvarez-Manilla, Maria Kamar, Intaek Lee, Jin-Kyu Lee, Karolyn, Troupe, Wei-Jie Zhang, Motoki Osawa and Michael Pierce “A novel β(1,6)-N acetylglucosaminyltransferase V (GnT-VB)” FEBS Letters2003, 554:515-519, https://doi.org/10.1016/s0014-5793(03)01234-1.

 

  1. Huabei Guo, Intaek Lee, Maria Kamar, and Michael Pierce “N-acetylglucosaminyltransferase V expression levels Regulate Cadherin-associated Homotypic cell-cell adhesion and Intracellular Signaling Pathways” Journal of Biological Chemistry2003, 278: 52412-52424, https://doi.org/10.1074/jbc.m308837200.

 

  1. Huabei Guo, Intaek Lee, Maria Kamar, Steven Akiyama and Michael Pierce “Aberrant N-glycosylation of β1 integrin causes reduced α5β1 integrin clustering and stimulates cell migration” Cancer Research2002, 62: 6837-6845. 

 

Mentoring and Training Experiences:

  • 2014-2015 Jia Mei (Former Technician) = PhD program at University of Munich, Germany
  • 2014-2016 Mengjing Bao (Former Technician) = PhD program in University of Bern, Switzerland
  • 2015-2018 Qiang Yue (MS student) = Pfizer, Pudong, China
  • 2015-2017 Xihua Yue (postdoc) = Research assistant professor, ShanghaiTech University

 

Invited and Conference Talks:

  1. American Society for Cell Biology/European Molecular Biology Organization 2018 meeting, Talk given in a minisymposia “Organelle Zone” San Diego, CA USA 2018
  2. American Society for Cell Biology annual meeting, Talk given in a minisymposia “New insights into Secretory Trafficking Mechanism” San Diego, CA USA 2015
  3. Korea Institute for Advanced Study (KIAS), Korea Advanced Institute of Science and Technology, Seoul, South Korea 2015
  4. Sungkyunkwan University, School of Pharmacy, Suwon, South Korea 2015

Reviewer activity for peer-reviewed journals

Small GTPases, Cellular and Molecular Life Sciences

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